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µg mouse α il 33  (Bio-Techne corporation)


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    Structured Review

    Bio-Techne corporation µg mouse α il 33
    ( A-C ) Female and male mice were infected with 10 7 CFU of UPEC UTI89-RFP-kan R and bladders analyzed at 24 hours PI. Graphs show ( A <t>)</t> <t>IL-33</t> protein levels in homogenized bladder, ( B ) IL-4Rα expression on bladder resident macrophages, and ( C ) the number of ILCs (CD90 + CD25 + CD3 − CD4 − NK1.1 − MHC II − CD11b − ) in bladders. ( D-F ) Female mice were implanted with empty tubing (Mock) or slow release tubing containing testosterone (T tube) and allowed to recover 1 week before infection with 10 7 CFU UPEC strain UTI89-RFP-kan R . Graphs show ( D ) IL-33 protein levels in homogenized bladder tissue, ( E ) IL-4Rα expression on bladder resident macrophages, and ( F ) the number of ILCs in bladders. Data are pooled from 2-3 experiments, n=4-5 mice/group in each experiment. Each dot is one mouse, red dots depict female mice and blue dots are male mice, lines are medians. ns = not significant, ** p<0.01, *** p<0.001, **** p<0.0001 Mann-Whitney test. Analyses in this figure were corrected for multiple testing by Holm–Bonferroni method, all p<0.05 had q< 0.05.
    µg Mouse α Il 33, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 97/100, based on 310 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%C2%B5g+mouse+%CE%B1+il+33/Mouse+IL-33+Antibody/bio_rxiv__449124-231-4-9
    Average 97 stars, based on 310 article reviews
    µg mouse α il 33 - by Bioz Stars, 2026-10
    97/100 stars

    Images

    1) Product Images from "Sex differences in IL-17 determine chronicity in male versus female urinary tract infection"

    Article Title: Sex differences in IL-17 determine chronicity in male versus female urinary tract infection

    Journal: bioRxiv

    doi: 10.1101/449124

    ( A-C ) Female and male mice were infected with 10 7 CFU of UPEC UTI89-RFP-kan R and bladders analyzed at 24 hours PI. Graphs show ( A ) IL-33 protein levels in homogenized bladder, ( B ) IL-4Rα expression on bladder resident macrophages, and ( C ) the number of ILCs (CD90 + CD25 + CD3 − CD4 − NK1.1 − MHC II − CD11b − ) in bladders. ( D-F ) Female mice were implanted with empty tubing (Mock) or slow release tubing containing testosterone (T tube) and allowed to recover 1 week before infection with 10 7 CFU UPEC strain UTI89-RFP-kan R . Graphs show ( D ) IL-33 protein levels in homogenized bladder tissue, ( E ) IL-4Rα expression on bladder resident macrophages, and ( F ) the number of ILCs in bladders. Data are pooled from 2-3 experiments, n=4-5 mice/group in each experiment. Each dot is one mouse, red dots depict female mice and blue dots are male mice, lines are medians. ns = not significant, ** p<0.01, *** p<0.001, **** p<0.0001 Mann-Whitney test. Analyses in this figure were corrected for multiple testing by Holm–Bonferroni method, all p<0.05 had q< 0.05.
    Figure Legend Snippet: ( A-C ) Female and male mice were infected with 10 7 CFU of UPEC UTI89-RFP-kan R and bladders analyzed at 24 hours PI. Graphs show ( A ) IL-33 protein levels in homogenized bladder, ( B ) IL-4Rα expression on bladder resident macrophages, and ( C ) the number of ILCs (CD90 + CD25 + CD3 − CD4 − NK1.1 − MHC II − CD11b − ) in bladders. ( D-F ) Female mice were implanted with empty tubing (Mock) or slow release tubing containing testosterone (T tube) and allowed to recover 1 week before infection with 10 7 CFU UPEC strain UTI89-RFP-kan R . Graphs show ( D ) IL-33 protein levels in homogenized bladder tissue, ( E ) IL-4Rα expression on bladder resident macrophages, and ( F ) the number of ILCs in bladders. Data are pooled from 2-3 experiments, n=4-5 mice/group in each experiment. Each dot is one mouse, red dots depict female mice and blue dots are male mice, lines are medians. ns = not significant, ** p<0.01, *** p<0.001, **** p<0.0001 Mann-Whitney test. Analyses in this figure were corrected for multiple testing by Holm–Bonferroni method, all p<0.05 had q< 0.05.

    Techniques Used: Infection, Expressing, MANN-WHITNEY

    ( A ) Experimental design in which male mice (blue) were supplemented with recombinant IL-33 (rIL-33) or female mice (red) were treated with α-IL-33 neutralizing antibody. All mice were infected with 10 7 CFU UPEC and bacteriuria was assessed over time. ( B-G ) Graphs show ( B ) the number of infected male mice over time and ( C ) CFU at 24 hours post primary (1°) or challenge (2°) infection in the bladder, in PBS or rIL-33 treated male mice; ( D ) the number of infected mice over time and ( E ) CFU at 24 hours post primary (1°) or challenge (2°) infection in the bladder, in isotype or α-IL-33 treated female mice; and the absolute number of the ( F ) indicated immune cell populations and ( G ) UPEC + immune cell populations in bladders 24 hours PI in isotype or α-IL-33 treated female mice. Data in B and D are representative experiments of 2 independent experiments, n=6 mice/group/experiment. In C, E-G , data are pooled from 2 experiments, n=4-5 mice/group in each experiment, each dot is one mouse, lines are medians. Mann-Whitney test, no statistically significant differences found.
    Figure Legend Snippet: ( A ) Experimental design in which male mice (blue) were supplemented with recombinant IL-33 (rIL-33) or female mice (red) were treated with α-IL-33 neutralizing antibody. All mice were infected with 10 7 CFU UPEC and bacteriuria was assessed over time. ( B-G ) Graphs show ( B ) the number of infected male mice over time and ( C ) CFU at 24 hours post primary (1°) or challenge (2°) infection in the bladder, in PBS or rIL-33 treated male mice; ( D ) the number of infected mice over time and ( E ) CFU at 24 hours post primary (1°) or challenge (2°) infection in the bladder, in isotype or α-IL-33 treated female mice; and the absolute number of the ( F ) indicated immune cell populations and ( G ) UPEC + immune cell populations in bladders 24 hours PI in isotype or α-IL-33 treated female mice. Data in B and D are representative experiments of 2 independent experiments, n=6 mice/group/experiment. In C, E-G , data are pooled from 2 experiments, n=4-5 mice/group in each experiment, each dot is one mouse, lines are medians. Mann-Whitney test, no statistically significant differences found.

    Techniques Used: Recombinant, Infection, MANN-WHITNEY

    Related Articles

    Neutralization:

    Article Title: Sex differences in IL-17 determine chronicity in male versus female urinary tract infection
    Article Snippet: For IL-33 neutralization, 3.6 μg/mouse α-IL-33 (catalog number AF3626, BioTechne/R&D Systems) or 3.6 μg/mouse polyclonal goat IgG isotype control (catalog number AB-108-C, BioTechne/R&D Systems) were delivered intraperitoneally in 100 μL PBS ( ).

    Infection:

    Article Title: Sex differences in IL-17 determine chronicity in male versus female urinary tract infection
    Article Snippet: For IL-33 neutralization, 3.6 μg/mouse α-IL-33 (catalog number AF3626, BioTechne/R&D Systems) or 3.6 μg/mouse polyclonal goat IgG isotype control (catalog number AB-108-C, BioTechne/R&D Systems) were delivered intraperitoneally in 100 μL PBS ( ).

    Expressing:

    Article Title: Sex differences in IL-17 determine chronicity in male versus female urinary tract infection
    Article Snippet: For IL-33 neutralization, 3.6 μg/mouse α-IL-33 (catalog number AF3626, BioTechne/R&D Systems) or 3.6 μg/mouse polyclonal goat IgG isotype control (catalog number AB-108-C, BioTechne/R&D Systems) were delivered intraperitoneally in 100 μL PBS ( ).

    MANN-WHITNEY:

    Article Title: Sex differences in IL-17 determine chronicity in male versus female urinary tract infection
    Article Snippet: For IL-33 neutralization, 3.6 μg/mouse α-IL-33 (catalog number AF3626, BioTechne/R&D Systems) or 3.6 μg/mouse polyclonal goat IgG isotype control (catalog number AB-108-C, BioTechne/R&D Systems) were delivered intraperitoneally in 100 μL PBS ( ).

    Recombinant:

    Article Title: Sex differences in IL-17 determine chronicity in male versus female urinary tract infection
    Article Snippet: For IL-33 neutralization, 3.6 μg/mouse α-IL-33 (catalog number AF3626, BioTechne/R&D Systems) or 3.6 μg/mouse polyclonal goat IgG isotype control (catalog number AB-108-C, BioTechne/R&D Systems) were delivered intraperitoneally in 100 μL PBS ( ).



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    Bio-Techne corporation µg mouse α il 33
    ( A-C ) Female and male mice were infected with 10 7 CFU of UPEC UTI89-RFP-kan R and bladders analyzed at 24 hours PI. Graphs show ( A <t>)</t> <t>IL-33</t> protein levels in homogenized bladder, ( B ) IL-4Rα expression on bladder resident macrophages, and ( C ) the number of ILCs (CD90 + CD25 + CD3 − CD4 − NK1.1 − MHC II − CD11b − ) in bladders. ( D-F ) Female mice were implanted with empty tubing (Mock) or slow release tubing containing testosterone (T tube) and allowed to recover 1 week before infection with 10 7 CFU UPEC strain UTI89-RFP-kan R . Graphs show ( D ) IL-33 protein levels in homogenized bladder tissue, ( E ) IL-4Rα expression on bladder resident macrophages, and ( F ) the number of ILCs in bladders. Data are pooled from 2-3 experiments, n=4-5 mice/group in each experiment. Each dot is one mouse, red dots depict female mice and blue dots are male mice, lines are medians. ns = not significant, ** p<0.01, *** p<0.001, **** p<0.0001 Mann-Whitney test. Analyses in this figure were corrected for multiple testing by Holm–Bonferroni method, all p<0.05 had q< 0.05.
    µg Mouse α Il 33, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%C2%B5g+mouse+%CE%B1+il+33/Mouse+IL-33+Antibody/bio_rxiv__449124-231-4-9
    Average 97 stars, based on 1 article reviews
    µg mouse α il 33 - by Bioz Stars, 2026-10
    97/100 stars
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    ( A-C ) Female and male mice were infected with 10 7 CFU of UPEC UTI89-RFP-kan R and bladders analyzed at 24 hours PI. Graphs show ( A ) IL-33 protein levels in homogenized bladder, ( B ) IL-4Rα expression on bladder resident macrophages, and ( C ) the number of ILCs (CD90 + CD25 + CD3 − CD4 − NK1.1 − MHC II − CD11b − ) in bladders. ( D-F ) Female mice were implanted with empty tubing (Mock) or slow release tubing containing testosterone (T tube) and allowed to recover 1 week before infection with 10 7 CFU UPEC strain UTI89-RFP-kan R . Graphs show ( D ) IL-33 protein levels in homogenized bladder tissue, ( E ) IL-4Rα expression on bladder resident macrophages, and ( F ) the number of ILCs in bladders. Data are pooled from 2-3 experiments, n=4-5 mice/group in each experiment. Each dot is one mouse, red dots depict female mice and blue dots are male mice, lines are medians. ns = not significant, ** p<0.01, *** p<0.001, **** p<0.0001 Mann-Whitney test. Analyses in this figure were corrected for multiple testing by Holm–Bonferroni method, all p<0.05 had q< 0.05.

    Journal: bioRxiv

    Article Title: Sex differences in IL-17 determine chronicity in male versus female urinary tract infection

    doi: 10.1101/449124

    Figure Lengend Snippet: ( A-C ) Female and male mice were infected with 10 7 CFU of UPEC UTI89-RFP-kan R and bladders analyzed at 24 hours PI. Graphs show ( A ) IL-33 protein levels in homogenized bladder, ( B ) IL-4Rα expression on bladder resident macrophages, and ( C ) the number of ILCs (CD90 + CD25 + CD3 − CD4 − NK1.1 − MHC II − CD11b − ) in bladders. ( D-F ) Female mice were implanted with empty tubing (Mock) or slow release tubing containing testosterone (T tube) and allowed to recover 1 week before infection with 10 7 CFU UPEC strain UTI89-RFP-kan R . Graphs show ( D ) IL-33 protein levels in homogenized bladder tissue, ( E ) IL-4Rα expression on bladder resident macrophages, and ( F ) the number of ILCs in bladders. Data are pooled from 2-3 experiments, n=4-5 mice/group in each experiment. Each dot is one mouse, red dots depict female mice and blue dots are male mice, lines are medians. ns = not significant, ** p<0.01, *** p<0.001, **** p<0.0001 Mann-Whitney test. Analyses in this figure were corrected for multiple testing by Holm–Bonferroni method, all p<0.05 had q< 0.05.

    Article Snippet: For IL-33 neutralization, 3.6 µg/mouse α-IL-33 (catalog number AF3626, BioTechne/R&D Systems) or 3.6 µg/mouse polyclonal goat IgG isotype control (catalog number AB-108-C, BioTechne/R&D Systems) were delivered intraperitoneally in 100 µL PBS ( ).

    Techniques: Infection, Expressing, MANN-WHITNEY

    ( A ) Experimental design in which male mice (blue) were supplemented with recombinant IL-33 (rIL-33) or female mice (red) were treated with α-IL-33 neutralizing antibody. All mice were infected with 10 7 CFU UPEC and bacteriuria was assessed over time. ( B-G ) Graphs show ( B ) the number of infected male mice over time and ( C ) CFU at 24 hours post primary (1°) or challenge (2°) infection in the bladder, in PBS or rIL-33 treated male mice; ( D ) the number of infected mice over time and ( E ) CFU at 24 hours post primary (1°) or challenge (2°) infection in the bladder, in isotype or α-IL-33 treated female mice; and the absolute number of the ( F ) indicated immune cell populations and ( G ) UPEC + immune cell populations in bladders 24 hours PI in isotype or α-IL-33 treated female mice. Data in B and D are representative experiments of 2 independent experiments, n=6 mice/group/experiment. In C, E-G , data are pooled from 2 experiments, n=4-5 mice/group in each experiment, each dot is one mouse, lines are medians. Mann-Whitney test, no statistically significant differences found.

    Journal: bioRxiv

    Article Title: Sex differences in IL-17 determine chronicity in male versus female urinary tract infection

    doi: 10.1101/449124

    Figure Lengend Snippet: ( A ) Experimental design in which male mice (blue) were supplemented with recombinant IL-33 (rIL-33) or female mice (red) were treated with α-IL-33 neutralizing antibody. All mice were infected with 10 7 CFU UPEC and bacteriuria was assessed over time. ( B-G ) Graphs show ( B ) the number of infected male mice over time and ( C ) CFU at 24 hours post primary (1°) or challenge (2°) infection in the bladder, in PBS or rIL-33 treated male mice; ( D ) the number of infected mice over time and ( E ) CFU at 24 hours post primary (1°) or challenge (2°) infection in the bladder, in isotype or α-IL-33 treated female mice; and the absolute number of the ( F ) indicated immune cell populations and ( G ) UPEC + immune cell populations in bladders 24 hours PI in isotype or α-IL-33 treated female mice. Data in B and D are representative experiments of 2 independent experiments, n=6 mice/group/experiment. In C, E-G , data are pooled from 2 experiments, n=4-5 mice/group in each experiment, each dot is one mouse, lines are medians. Mann-Whitney test, no statistically significant differences found.

    Article Snippet: For IL-33 neutralization, 3.6 µg/mouse α-IL-33 (catalog number AF3626, BioTechne/R&D Systems) or 3.6 µg/mouse polyclonal goat IgG isotype control (catalog number AB-108-C, BioTechne/R&D Systems) were delivered intraperitoneally in 100 µL PBS ( ).

    Techniques: Recombinant, Infection, MANN-WHITNEY