µg mouse α il 33 (Bio-Techne corporation)
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µg Mouse α Il 33, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 97/100, based on 310 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%C2%B5g+mouse+%CE%B1+il+33/Mouse+IL-33+Antibody/bio_rxiv__449124-231-4-9
Average 97 stars, based on 310 article reviews
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1) Product Images from "Sex differences in IL-17 determine chronicity in male versus female urinary tract infection"
Article Title: Sex differences in IL-17 determine chronicity in male versus female urinary tract infection
Journal: bioRxiv
doi: 10.1101/449124
Figure Legend Snippet: ( A-C ) Female and male mice were infected with 10 7 CFU of UPEC UTI89-RFP-kan R and bladders analyzed at 24 hours PI. Graphs show ( A ) IL-33 protein levels in homogenized bladder, ( B ) IL-4Rα expression on bladder resident macrophages, and ( C ) the number of ILCs (CD90 + CD25 + CD3 − CD4 − NK1.1 − MHC II − CD11b − ) in bladders. ( D-F ) Female mice were implanted with empty tubing (Mock) or slow release tubing containing testosterone (T tube) and allowed to recover 1 week before infection with 10 7 CFU UPEC strain UTI89-RFP-kan R . Graphs show ( D ) IL-33 protein levels in homogenized bladder tissue, ( E ) IL-4Rα expression on bladder resident macrophages, and ( F ) the number of ILCs in bladders. Data are pooled from 2-3 experiments, n=4-5 mice/group in each experiment. Each dot is one mouse, red dots depict female mice and blue dots are male mice, lines are medians. ns = not significant, ** p<0.01, *** p<0.001, **** p<0.0001 Mann-Whitney test. Analyses in this figure were corrected for multiple testing by Holm–Bonferroni method, all p<0.05 had q< 0.05.
Techniques Used: Infection, Expressing, MANN-WHITNEY
Figure Legend Snippet: ( A ) Experimental design in which male mice (blue) were supplemented with recombinant IL-33 (rIL-33) or female mice (red) were treated with α-IL-33 neutralizing antibody. All mice were infected with 10 7 CFU UPEC and bacteriuria was assessed over time. ( B-G ) Graphs show ( B ) the number of infected male mice over time and ( C ) CFU at 24 hours post primary (1°) or challenge (2°) infection in the bladder, in PBS or rIL-33 treated male mice; ( D ) the number of infected mice over time and ( E ) CFU at 24 hours post primary (1°) or challenge (2°) infection in the bladder, in isotype or α-IL-33 treated female mice; and the absolute number of the ( F ) indicated immune cell populations and ( G ) UPEC + immune cell populations in bladders 24 hours PI in isotype or α-IL-33 treated female mice. Data in B and D are representative experiments of 2 independent experiments, n=6 mice/group/experiment. In C, E-G , data are pooled from 2 experiments, n=4-5 mice/group in each experiment, each dot is one mouse, lines are medians. Mann-Whitney test, no statistically significant differences found.
Techniques Used: Recombinant, Infection, MANN-WHITNEY
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Neutralization:Article Title: Sex differences in IL-17 determine chronicity in male versus female urinary tract infection Article Snippet: For IL-33 neutralization, 3.6 μg/mouse α-IL-33 (catalog number AF3626, BioTechne/R&D Systems) or 3.6 μg/mouse polyclonal goat IgG isotype control ( Infection:Article Title: Sex differences in IL-17 determine chronicity in male versus female urinary tract infection Article Snippet: For IL-33 neutralization, 3.6 μg/mouse α-IL-33 (catalog number AF3626, BioTechne/R&D Systems) or 3.6 μg/mouse polyclonal goat IgG isotype control ( Expressing:Article Title: Sex differences in IL-17 determine chronicity in male versus female urinary tract infection Article Snippet: For IL-33 neutralization, 3.6 μg/mouse α-IL-33 (catalog number AF3626, BioTechne/R&D Systems) or 3.6 μg/mouse polyclonal goat IgG isotype control ( MANN-WHITNEY:Article Title: Sex differences in IL-17 determine chronicity in male versus female urinary tract infection Article Snippet: For IL-33 neutralization, 3.6 μg/mouse α-IL-33 (catalog number AF3626, BioTechne/R&D Systems) or 3.6 μg/mouse polyclonal goat IgG isotype control ( Recombinant:Article Title: Sex differences in IL-17 determine chronicity in male versus female urinary tract infection Article Snippet: For IL-33 neutralization, 3.6 μg/mouse α-IL-33 (catalog number AF3626, BioTechne/R&D Systems) or 3.6 μg/mouse polyclonal goat IgG isotype control ( |